anti-LPAR6 antibody product blog
Tags: Antibody; Polyclonal Antibody; LPAR6; anti-LPAR6 antibody;
The LPAR6 lpar6 (Catalog #MBS9212496) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The LPAR6 Antibody (Center) reacts with Human, Mouse, RatPredicted: Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s LPAR6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) - Paraffin, ELISA (EIA).
WB~~1:1000
IHC-P~~1:10~50. Researchers should empirically determine the suitability of the LPAR6 lpar6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The LPAR6 lpar6 product has the following accession number(s) (GI #241982706) (NCBI Accession #NP_001155969.1) (Uniprot Accession #P43657). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the LPAR6 Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (All lanes : Anti-LPAR6 Antibody (Center) at 1:2000 dilution
Lane 1: Molt-4 whole cell lysate
Lane 2: HepG2 whole cell lysate
Lane 3: Mouse heart tissue lysate
Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 39 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (All lanes : Anti-LPAR6 Antibody (Center) at 1:1000 dilution
Lane 1: Mouse heart tissue lysate
Lane 2: Rat heart tissue lysate
Lysates/proteins at 20 ug per lane.
Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution.
Predicted band size : 39 kDa
Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) (LPAR6 Antibody (Center) (MBS9212496) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the LPAR6 antibody detected the LPAR6 protein (arrow).)
Immunohostochemistry (IHC) (LPAR6 Antibdy (Center) (MBS9212496) immunohistochemistry analysis in formalin fixed and paraffin embedded human breast carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of LPAR6 Antibdy (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
The protein encoded by this gene belongs to the family of G-protein coupled receptors, that are preferentially activated by adenosine and uridine nucleotides. This gene aligns with an internal intron of the retinoblastoma susceptibility gene in the reverse orientation. Alternative splicing results in multiple transcript variants.
Function: Binds to oleoyl-L-alpha-lysophosphatidic acid (LPA). Intracellular cAMP is involved in the receptor activation. Important for the maintenance of hair growth and texture.
Cellular Location: Cell membrane; Multi-pass membrane protein
Tissue Location: Expressed ubiquitously, including in skin and hair follicle cells. Detected in both Henle\'s and Huxley\'s layers of the inner root sheath of the hair follicle and in suprabasal layers of the epidermis (at protein level). Expressed at low levels in peripheral blood leukocytes.