anti-MAK antibody product blog
Tags: Antibody; Polyclonal Antibody; Mak; anti-MAK antibody;
The MAK mak (Catalog #MBS9205392) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAK Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MAK can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Western Blot (WB).WB~~1:1000. Researchers should empirically determine the suitability of the MAK mak for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MAK mak product has the following accession number(s) (GI #334724436) (NCBI Accession #NP_001229314.1) (Uniprot Accession #P20794). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MAK Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MAK (arrow) using MAK Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MAK gene (Lane 2) (Origene Technologies).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (MAK Antibody (T593) western blot analysis in Y79 cell line lysates (35ug/lane).This demonstrates the MAK antibody detected the MAK protein (arrow).)
MAK is a serine/threonine protein kinase related to kinases involved in cell cycle regulation. It is expressed almost exclusively in the testis, primarily in germ cells. Studies of the mouse and rat homologs have localized the kinase to the chromosomes during meiosis in spermatogenesis, specifically to the synaptonemal complex that exists while homologous chromosomes are paired. There is, however, a study of the mouse homolog that has identified high levels of expression in developing sensory epithelia so its function may be more generalized.
Antigen Type: Synthetic Peptide. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAK are readily searchable from our website. Different antibodies against the same target such as MAK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.