anti-Man2a2 antibody product blog
Tags: Antibody; Polyclonal Antibody; MAN2A2; anti-Man2a2 antibody;
The Man2a2 man2a2 (Catalog #MBS649508) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MAN2A2, ID (MAN2A2, MANA2X, Alpha-mannosidase 2x, Alpha-mannosidase IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s MAN2A2 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).Suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, ELISA
Dilution: ELISA: 1:1,000
Western Blot: 1:100-500
Immunohistochemistry: 1:10-50
Flow Cytometry: 1:10-50. Researchers should empirically determine the suitability of the Man2a2 man2a2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The Man2a2 man2a2 product has the following accession number(s) (GI #32822711) (NCBI Accession #AAH55006.1) (Uniprot Accession #Q8BRK9). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MAN2A2, ID (MAN2A2, MANA2X, Alpha-mannosidase 2x, Alpha-mannosidase IIx, Mannosidase alpha class 2A member 2, Mannosyl-oligosaccharide 1,3-1,6-alpha-mannosidase) with the following immunoassay(s):
Western Blot (WB) (MAN2A2 Antibody (Center) western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the MAN2A2 antibody detected the MAN2A2 protein (arrow).)
Immunohistochemistry (IHC) (MAN2A2 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of MAN2A2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (MAN2A2 Antibody (Center) flow cytometric analysis of Neuro-2a cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)
MAN2A2 catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway.
Immunogen: MAN2A2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 766-796 amino acids from the Central region of human MAN2A2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Man2a2 are readily searchable from our website. Different antibodies against the same target such as Man2a2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.