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anti-MAP3K3 antibody product blog

Posted on 2015-11-24 00:04:50 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-MAP3K3 antibody; MAP3K3; MAP3K3 / MEKK3;
The MAP3K3 map3k3 (Catalog #MBS246954) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal to Mouse MAP3K3 / MEKK3 reacts with Human, Mouse, Rat
Predicted Reactivity: Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken, Xenopus (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s MAP3K3 / MEKK3 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB).
IHC-P (1:100), WB (1:5000)
Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti.. Researchers should empirically determine the suitability of the MAP3K3 map3k3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MAP3K3 map3k3 product has the following accession number(s) (GI #42794765) (NCBI Accession #NP_002392.2) (Uniprot Accession #Q99759). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal to Mouse MAP3K3 / MEKK3 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-MAP3K3 / MEKK3 antibody IHC of human uterus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC) MAP3K3.

MEKK3, a MAP3K type protein kinase, is a member of several MAP kinase signaling pathways. It regulates both JNK and ERK cascades by phosphorylating and activating MKK4/MAP2K4 and MEK1/MAP2K1, respectively. MEKK3 regulates the ERK5/MAPK7 cascade by phosphorylating MEK5 and the p38 MAP kinase cascade by phosphorylating MEK3/MAP2K3 and MKK6/MAP2K6.

Target Species: Mouse
Immunogen Description: A 17 residue synthetic peptide DEQEALDSIMKDLVALQ(C) based on the mouse MekK3 (residues 2-18) with the cysteine (C) residue added and the peptide coupled to KLH. Immunogen Type: Synthetic peptide - KLH conjugated
Immunogen: MAP3K3 / MEKK3 antibody was raised against a 17 residue synthetic peptide DEQEALDSIMKDLVALQ(C) based on the mouse MekK3 (residues 2-18) with the cysteine (C) residue added and the peptide coupled to KLH. Percent identity by BLAST analysis: Mouse, Rat, Elephant (100%); Panda, Bovine, Dog, Bat, Horse, Rabbit, Pig, Opossum, Turkey, Chicken, Platypus, Lizard, Xenopus (94%); Human, Gorilla, Gibbon, Monkey (88%); Stickleback, Pufferfish (82%). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAP3K3 are readily searchable from our website. Different antibodies against the same target such as MAP3K3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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