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anti-MAPK14 antibody product blog

Posted on 2015-10-21 23:36:09 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-MAPK14 antibody; MAPK14; MAPK14-T180/Y182;
The MAPK14 mapk14 (Catalog #MBS9128155) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MAPK14-T180/Y182 Polyclonal Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MAPK14-T180/Y182 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).
WB 1:500 - 1:2000. Researchers should empirically determine the suitability of the MAPK14 mapk14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MAPK14 mapk14 product has the following accession number(s) (GI #4503069) (NCBI Accession #NP_001306.1) (Uniprot Accession #Q16539). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Phospho-MAPK14-T180/Y182 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using Phospho-MAPK14-T180/Y182 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% BSA.)
Western Blot (WB) MAPK14.

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response. Four alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported.

Route: Synthetic Peptide. Immunogen: A phospho specific peptide corresponding to residues surrounding T180/Y182 of human MAPK14. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MAPK14 are readily searchable from our website. Different antibodies against the same target such as MAPK14 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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