anti-MARCKS antibody product blog
Tags: Antibody; Polyclonal Antibody; MARCKS; anti-MARCKS antibody;
The MARCKS marcks (Catalog #MBS840440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Phospho-MARCKS Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s MARCKS can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Western blotting (1:200-1000). However, the optimal conditions should be determined individually. Other applications have not been determined. The antibody detects 80 kDa MARCKS only when phosphorylated at Ser152/156. Researchers should empirically determine the suitability of the MARCKS marcks for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MARCKS marcks product has the following accession number(s) (GI #266495) (NCBI Accession #P30009.2) (Uniprot Accession #P30009). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Background: MARCKS (Myristoylated alanine-rich protein kinase C substrate) is a major PKC substrate that is distributed in various cell types. MARCKS has been implicated in cell motility, cell adhesion, phagocytosis, membrane traffic and mitogenesis. PKC phosphorylates Ser152, 156 and 163 of MARCKS, which regulates MARCKS\'s calcium/calmodulin binding activity and filamentous (F)-actin cross-linking activity. In addition, phosphorylation by PKC results in translocation of MARCKS from membrane to cytoplasm.
Immunogen: Synthetic peptide corresponding to amino acids surrounding Ser152/156 of rat MARCKS. Handling: The antibody solution should be gently mixed before use. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MARCKS are readily searchable from our website. Different antibodies against the same target such as MARCKS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Brain, Dorsal Root Ganglion, Embryonic Tissue, Eye, Heart, Lung, Muscle, Nerve, Ovary, Pancreas tissues are correlated with this protein. MARCKS also interacts with the following gene(s): Apoe, Cdk2, Mlp, Prkca, Prkcd, Prkce, Prkcg. The following patways have been known to be associated with this gene.