anti-MCM5 antibody product blog
Tags: Antibody; Polyclonal Antibody; MCM5; anti-Mcm5 antibody;
The MCM5 mcm5 (Catalog #MBS175634) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM5 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s MCM5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the MCM5 mcm5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The MCM5 mcm5 product has the following accession number(s) (GI #19858646) (NCBI Accession #P33992.5) (Uniprot Accession #P33992). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-MCM5 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-MCM5 antibody, MBS175634, Western blotting
Lane 1: Rat Testis Tissue Lysate
Lane 2: Rat Brain Tissue Lysate Lane3: JURKAT Cell Lysate
)
Immunohistochemistry (IHC) (Anti-MCM5 antibody, MBS175634, IHC(P)
IHC(P): Human Intestinal Cancer Tissue )
Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM5(MCM5) detection. Tested with WB, IHC-P in Human, Mouse, Rat.
Background: MCM5(MINICHROMOSOME MAINTENANCE, S. CEREVISIAE, HOMOLOG OF, 5),also known as CDC46, is a protein that in humans is encoded by the MCM5 gene. MCM5 is structurally very similar to the CDC46 protein from S. cerevisiae, a protein involved in the initiation of DNA replication. And MCM5 is a member of the MCM family of chromatin-binding proteins and can interact with at least two other members of this family. The MCM5 gene is mapped on 22q12.3. The MCM5 gene contains 16 exons. The encoded protein is upregulated in the transition from the G0 to G1/S phase of the cell cycle and may actively participate in cell cycle regulation.
Immunogen: A synthetic peptide corresponding to a sequence at the C-terminus of human MCM5 (720-734aa EIQHRMQRKVLYRLK), identical to the related rat and mouse sequences. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MCM5 are readily searchable from our website. Different antibodies against the same target such as MCM5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.