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anti-MCM6 antibody product blog

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Posted on 2015-07-19 21:51:32 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; MCM6; anti-MCM6 antibody;
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The MCM6 mcm6 (Catalog #MBS175395) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-MCM6 antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s MCM6 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin, Immunohistochemistry (IHC) Formalin, Immunocytochemistry ICC. Researchers should empirically determine the suitability of the MCM6 mcm6 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MCM6 mcm6 product has the following accession number(s) (GI #2497824) (NCBI Accession #Q14566.1) (Uniprot Accession #Q14566). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-MCM6 antibody with the following immunoassay(s):
Western Blot (WB) (Anti-MCM6 antibody, MBS175395, Western blotting
Lane 1: U87 Cell Lysate
Lane 2: COLO320 Cell Lysate
Lane 3: HELA Cell Lysate
Lane 4: MCF-7 Cell Lysate
Lane 5: JURKAT Cell Lysate
)
Western Blot (WB) MCM6.

Immunohistochemistry (IHC) (Anti-MCM6 antibody, MBS175395, IHC(P)
IHC(P): Human Intestinal Cancer Tissue )
Immunohistochemistry (IHC) MCM6.

Immunohistochemistry (IHC) (Anti-MCM6 antibody, MBS175395, IHC(P)
IHC(P): Rat Brain Tissue )
Immunohistochemistry (IHC) MCM6.

Immunocytochemistry (ICC) (Anti-MCM6 antibody, MBS175395, ICC
ICC: HELA Cell )
Immunocytochemistry (ICC) MCM6.

Immunohistochemistry (IHC) (Anti-MCM6 antibody, MBS175395, IHC(F)
IHC(F): Human Placenta Tissue )
Immunohistochemistry (IHC) MCM6.

Description: Rabbit IgG polyclonal antibody for DNA replication licensing factor MCM6(MCM6) detection. Tested with WB, IHC-P, IHC-F, ICC in Human, Mouse, Rat.
Background: MCM6(Minichromosome maintenance, s. pombe, homolog of, 6) is a protein that in humans is encoded by the MCM6 gene. MCM6 is one of the highly conserved mini-chromosome maintenance proteins(MCM) that are essential for the initiation of eukaryotic genome replication. The MCM genes were originally identified in yeast defective in minichromosome maintenance and have since been shown to play roles in the progression of the cell cycle; many are cell division control genes. The MCM6 gene is mapped on 2q21.3. Mcm 6 has recently been shown to interact strongly Cdt1 at defined residues, by mutating these target residues Wei et al. observed lack of Cdt1 recruitment of Mcm2-7 to the pre-RC. An approximately 200-kb region surrounding the C/T(-13910) polymorphism in MCM6 intron 13 functioned as an enhancer of the lactase gene promoter in intestinal cell culture.

Immunogen: A synthetic peptide corresponding to a sequence in the middle region of human MCM6 (589-605aa ESEDFIVEQYKHLRQRD), different from the related rat and mouse sequences by one amino acid. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml.
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