anti-MDH1 antibody product blog
Tags: Antibody; Polyclonal Antibody; MDH1; anti-MDH1 antibody;
The MDH1 mdh1 (Catalog #MBS625398) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MDH1, CT (Malate Dehydrogenase, Cytoplasmic, Cytosolic Malate Dehydrogenase, MDHA) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MDH1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Suitable for use in ELISA and Western Blot.
Dilution: ELISA: 1:1,000
Western Blot: 1:50-1:100. Researchers should empirically determine the suitability of the MDH1 mdh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MDH1 mdh1 product has the following accession number(s) (GI #312283701) (NCBI Accession #NP_001186040.1) (Uniprot Accession #P40925). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MDH1, CT (Malate Dehydrogenase, Cytoplasmic, Cytosolic Malate Dehydrogenase, MDHA) with the following immunoassay(s):
Western Blot (WB) (MDH1 Antibody (C-term) western blot analysis in HL-60,THP-1 cell line lysates (35ug/lane).This demonstrates the MDH1 antibody detected the MDH1 protein (arrow).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MDH1 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (MDH1 Antibody (C-term) flow cytometry analysis of HL-60 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
MDH1 is localized to the cytoplasm and may play pivotal roles in the malate-aspartate shuttle that operates in the metabolic coordination between cytosol and mitochondria.
Immunogen: Synthetic peptide selected from the C-terminal region of human MDH1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MDH1 are readily searchable from our website. Different antibodies against the same target such as MDH1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Blood, Brain, Eye, Heart, Intestine, Kidney, Liver, Lung, Muscle, Nerve tissues are correlated with this protein.