anti-MDM2 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-MDM2 antibody; MDM2;
The MDM2 mdm2 (Catalog #MBS624935) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MDM2, phosphorylated (Ser166) (Murine Double Minute-2) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s MDM2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blotting.
Dilution: Western Blot: 1:1000. Researchers should empirically determine the suitability of the MDM2 mdm2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MDM2 mdm2 product has the following accession number(s) (GI #155183770) (Uniprot Accession #Q00987). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MDM2, phosphorylated (Ser166) (Murine Double Minute-2) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from COS cells untransfected, transfected with Wild-type MDM2 or with mutant MDM2 (S166S186A), using MBS624935)
Western Blot (WB) (Western blot analysis of extracts from IGF-1 treated MCF-7 cells, using MBS624935.)
MDM2, an ubiquitin ligase for p53, plays a central role in regulation of the stability of p53 (1) via Akt-mediated MDM2 phosphorylation at Ser166 and Ser186 (2,3). Phosphorylation of MDM2 increases its interaction with p300, providing a platform to allow the assembly of the protein complex necessary for MDM2-mediated ubiquitination and degradation of p53 (4). Phosphorylation of MDM2 also blocks its binding to p19ARF, increasing the degradation of p53 (3). Specificity/Sensitivity: Phospho-MDM2 (Ser166) antibody detects endogenous levels of MDM2 only when phosphorylated at serine 166.
Immunogen: Synthetic phosphopeptide (KLH coupled) corresponding to residues surrounding Ser166 of human MDM2. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MDM2 are readily searchable from our website. Different antibodies against the same target such as MDM2 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Liver, Lung, Lymph Node, Muscle, Prostate, Skin, Vascular tissues are correlated with this protein. Adenocarcinoma, Breast Neoplasms, Carcinoma, Cell Transformation, Neoplastic, Head and Neck Neoplasms, Leukemia, Lymphoma, Neoplasms, Neoplasms, Experimental, Neuroectodermal Tumors are some of the diseases may be linked to MDM2, phosphorylated (Ser166) (Murine Double Minute-2).