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anti-Med15 antibody product blog

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Posted on 2015-11-20 21:36:51 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; anti-MED15 antibody; MED15;
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The Med15 med15 (Catalog #MBS9202903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Med15 Antibody (C-term) reacts with Mouse, rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Med15 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA).
WB~~1:2000. Researchers should empirically determine the suitability of the Med15 med15 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The Med15 med15 product has the following accession number(s) (GI #20177999) (NCBI Accession #Q924H2.2) (Uniprot Accession #Q924H2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the (Mouse) Med15 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (Anti-Med15 Antibody (C-term) at 1:1000 dilution + mouse testis lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 87 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) Med15.

Western Blot (WB) (All lanes : Anti-Med15 Antibody (C-term) at 1:2000 dilution
Lane 1: mouse brain lysates
Lane 2: mouse spleen lysates
Lane 3: mouse thymus lysates
Lane 4: rat lung lysates

Lysates/proteins at 20 ug per lane.

Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution

Predicted band size : 87 kDa

Blocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB) Med15.

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors. Required for cholesterol-dependent gene regulation. Positively regulates the Nodal signaling pathway (By similarity).

Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Med15 are readily searchable from our website. Different antibodies against the same target such as Med15 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Med15 also interacts with the following gene(s): Cdk8, Med1, Med10, Med13, Med13l, Med14, Med18, Med6. Bone Marrow, Embryonic Tissue, Heart, Intestine, Kidney, Liver, Lung, Prostate, Skin, Uterus tissues are correlated with this protein.
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