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anti-MEF2C antibody product blog

Posted on 2016-01-09 03:20:45 by mybiosource_staff
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Tags: Antibody; MEF2C; anti-MEF2C antibody; Polyclonal Antibody;
The MEF2C mef2c (Catalog #MBS9204480) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MEF2C Antibody (S387) reacts with Human (Predicted Reactivity: Pig) and may cross-react with other species as described in the data sheet. MyBioSource\'s MEF2C can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunohistochemistry (IHC), Immunofluorescence (IF), Western Blot (WB).
WB~~1:1000. Researchers should empirically determine the suitability of the MEF2C mef2c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MEF2C mef2c product has the following accession number(s) (GI #196114945) (NCBI Accession #NP_001124477.1) (Uniprot Accession #Q06413). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MEF2C Antibody (S387) with the following immunoassay(s):
Western Blot (WB) (MEF2C Antibody (pS387) western blot analysis in HL-60,K562 cell line lysates (35ug/lane).This demonstrates the MEF2C antibody detected the MEF2C protein (arrow).)
Western Blot (WB) MEF2C.

Immunofluorescence (IF) (Fluorescent confocal image of Hela cell stained with MEF2C Antibody (S387). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with MEF2C primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min).MEF2C immunoreactivity is localized to vesicles and Nucleus significantly.)
Immunofluorescence (IF) MEF2C.

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human brain tissue reacted with MEF2C Antibody (S387), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Immunohistochemistry (IHC) MEF2C.

MEF2C is a transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. This protein controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. It may also be involved in neurogenesis and in the development of cortical architecture.

Antigen Type: Synthetic Peptide
Crown Antibody: Yes. Antigen Source: HUMAN. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MEF2C are readily searchable from our website. Different antibodies against the same target such as MEF2C may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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