|The Melanin Concentrating Hormone Receptor1 n/a (Catalog #MBS611370) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Melanin Concentrating Hormone Receptor1 (MCHR1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Melanin Concentrating Hormone Receptor1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1:1000-1:5000 using ECL.
ELISA: 1:10,000-1:100,000 using 50-100ng of control peptide/well. Researchers should empirically determine the suitability of the Melanin Concentrating Hormone Receptor1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The cyclic neuropeptide, Melanin-Concentrating Hormone (MCH), plays an important role in food intake and energy balance. MCH stimulates feeding. it is up regulated in obese mice and during fasting. Animals lacking MCH eat less and are leaner. A receptor for MCH (MCHR) has been cloned. Human MCH receptor is 402aa membrane protein described as an orphan G-protein coupled receptor SLC-1 or GPR24, somatostatin receptor-like protein. It has 7 transmembrane domains, a Dry motif at the boundary of TM3 and the 2nd intracellular loop, a consensus site for N-linked glycosylation at the N-terminus. MCHR is expressed in ventromedial and dorsomedial nuclei of the hypothalamus. An orphan G-protein coupled receptor (SLC-1, GPR24) has been identified as the receptor of MCH. MCH receptor (MCH1R; human 402aa, rat 353aa) is predicted to contain 7 transmembrane domains. Moderate levels of MCHR are also found in eye and skeletal muscle, tongue and pituitary. MCHR binds MCH with sub-nanomolar affinity. It is stimulated by MCH to mobilize intracellular Ca2+ and reduce forskolin-elevated cAMP levels. A second human MCH receptor (MCH2R) has been cloned and characterized. MCH2R gene encodes a 340aa protein with 38% identity with MCH1R. It has the basic characteristics of GPCR and belongs to class 1 GPCR superfamily. MCH2R transfected cells responds to MCH with an increase in intracellular Ca2+ levels and increased cellular extrusion of protons.
Immunogen: Synthetic peptide (16aa) near the cytoplasmic C-terminus of human MCHR/GPR24/SLC-1 (KLH). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Melanin Concentrating Hormone Receptor1 are readily searchable from our website. Different antibodies against the same target such as Melanin Concentrating Hormone Receptor1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.