anti-MFAP5 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-MFAP5 antibody; MFAP5;
The MFAP5 mfap5 (Catalog #MBS9204084) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MFAP5 Antibody (C-term) reacts with Human (Predicted Reactivity: Bovine, Mouse) and may cross-react with other species as described in the data sheet. MyBioSource\'s MFAP5 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P), Flow Cytometry (FC/FACS).WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50. Researchers should empirically determine the suitability of the MFAP5 mfap5 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MFAP5 mfap5 product has the following accession number(s) (GI #4505089) (NCBI Accession #NP_003471.1) (Uniprot Accession #Q13361). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MFAP5 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MFAP5 antibody (C-term) in K562 cell line lysates (35ug/lane). MFAP5 (arrow) was detected using the purified Pab.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human Skeletal muscle reacted with MFAP5 Antibody (C-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (MFAP5 Antibody (C-term) flow cytometry analysis of K562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Western Blot (WB) (Western blot analysis of MFAP5 (arrow) using rabbit polyclonal MFAP5 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MFAP5 gene.)
MFAP5 is a 25-kD microfibril-associated glycoprotein which is rich in serine and threonine residues. The protein lacks a hydrophobic carboxyl terminus and proline-, glutamine-, and tyrosine-rich regions, which are characteristics of a related 31-kDa microfibril-associated glycoprotein (MFAP2). The close similarity between these two proteins is confined to a central region of 60 aa where precise alignment of 7 cysteine residues occurs. The structural differences suggest that this protein has some functions that are distinct from those of MFAP2.
Function: May play a role in hematopoiesis. In the cardiovascular system, could regulate growth factors or participate in cell signaling in maintaining large vessel integrity (By similarity). Component of the elastin-associated microfibrils (PubMed:8557636).
Calculated MW: 19612. Cellular Location: Secreted, extracellular space, extracellular matrix. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MFAP5 are readily searchable from our website. Different antibodies against the same target such as MFAP5 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.