anti-MHCC antibody product blog
Tags: Antibody; Polyclonal Antibody; Major Histocompatibility Complex Class I C; MHCC; anti-MHCC antibody;
The MHCC hla-c (Catalog #MBS2026667) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Major Histocompatibility Complex Class I C (MHCC) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Major Histocompatibility Complex Class I C can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA).Western blotting: 1-5ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the MHCC hla-c for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MHCC hla-c product has the following accession number(s) (GI #52630342) (NCBI Accession #NP_002108.4) (Uniprot Accession #P10321). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- SVG YVDDTQFVRF DSDAASPRGE PRAPWVEQEG PEYWDRETQK YKRQAQADRV SLRNLRGYYN QSEDGSHTLQ RMSGCDLGPD GRLLRGYDQS AYDGKDYIAL NEDLRSWTAA DTAAQITQRK LEAARAAEQL RAYLEGTCVE WLRRYLENGK ETLQRAEPPK THVTHHPLSD HEATLRCWAL GFYPAEITLT WQRDGEDQTQ DTELVETRPA GDGTFQKWAA VVVPSGQEQR.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Major Histocompatibility Complex Class I C (MHCC) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Lane1: Human Lymphocytes Cells; Lane2: Human A431 Cells; Lane3: Porcine Liver Tissue.)
Western Blot (WB) (Western Blot: Sample: Recombinant MHCC, Human.)
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Prostate Gland Tissue.)
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Lung Cancer Tissue)
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Kidney Tissue)
Knockout Validation (Knockout Validation: Lane 1: Wild-type A431 cell lysate; Lane 2: MHCC knockout A431 cell lysate; Predicted MW: 41kd Observed MW: 48kd Primary Ab: 2ug/ml Rabbit Anti-Human MHCC Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Fragment: MHCC (Ser48~Thr305)
Organism Species: Homo sapiens (Human)
Cross Reactivity: Human, Pig. Conjugate: No Conjugate
Immunogen: Recombinant MHCC (Ser48~Thr305) expressed in E Coli.
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2062965. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MHCC are readily searchable from our website. Different antibodies against the same target such as MHCC may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.