anti-MIP-3 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-MIP-3 antibody; MIP-3;
The MIP-3 ccl23 (Catalog #MBS691232) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Anti-Human MIP-3 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MIP-3 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Neutr.Western Blot: To detect human MIP-3 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 g/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant human MIP-3 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions. Researchers should empirically determine the suitability of the MIP-3 ccl23 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MIP-3 ccl23 product has the following accession number(s) (GI #22538806) (NCBI Accession #NP_005055.2) (Uniprot Accession #P55773). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
MIP-3 is a CC chemokine that signals through the CCR1 receptor. MIP-3 chemoattracts monocytes, resting T-lymphocytes and neutrophils, but does not chemoattract activated lymphocytes. Additionally, MIP-3 has been shown to inhibit colony formation of bone marrow myeloid immature progenitors. Recombinant human MIP-3 is an 11.3 kDa protein containing 99 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines.
Antibody Generation: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant human MIP 3 (Macrophage Inflammatory Protein-3). Anti-human MIP 3 specific antibody was purified by affinity chromatography employing immobilized human MIP 3 matrix. Buffer: PBS; pH 7.2
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of human MIP-3 (100.0 ng/ml), a concentration of 2.0 - 4.0 g/ml of this antibody is required. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MIP-3 are readily searchable from our website. Different antibodies against the same target such as MIP-3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Bone Neoplasms, Carcinoma, Cardiovascular Diseases, Fatty Liver, Fetal Resorption, Leukemia, Myeloid, Acute, Obstetric Labor, Premature, Premature Birth, Retinoblastoma are some of the diseases may be linked to Rabbit Anti-Human MIP-3. The following patways have been known to be associated with this gene. Liver, Lung, Placenta, Uncharacterized Tissue tissues are correlated with this protein.