anti-MMP3 antibody product blog
Tags: Antibody; Polyclonal Antibody; MMP3; anti-MMP3 antibody;
The MMP3 mmp3 (Catalog #MBS127685) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MMP3 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s MMP3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the MMP3 mmp3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MMP3 mmp3 product has the following accession number(s) (GI #116857) (NCBI Accession #P08254.2) (Uniprot Accession #P08254). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MMP3 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using MMP3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP\'s are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades fibronectin, laminin, collagens III, IV, IX, and X, and cartilage proteoglycans. The enzyme is thought to be involved in wound repair, progression of atherosclerosis, and tumor initiation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq, Jul 2008].
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human MMP3
Calculated Molecular Weight: 54kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MMP3 are readily searchable from our website. Different antibodies against the same target such as MMP3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. MMP3 also interacts with the following gene(s): COL18A1, CTNNB1, EGFR, IL1B, MMP1, PLG, TIMP1, TNC, VEGFA.