anti-MOTS-c antibody product blog
Tags: Antibody; Polyclonal Antibody; MOTS-c; anti-MOTS-c antibody;
The MOTS-c mt-rnr1 (Catalog #MBS542112) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s MOTS-c can be used in a range of immunoassay formats including, but not limited to, ELISA, Immunoprecipitation (IP), Western Blot (WB).Dot Blot: 1:10,000
ELISA: 1:10,000
Immunoprecipitation: 1:200
Western Blot: 1:500
Dilutions are for reference only. Applications are not necessarily precluded from working with this antibody. Researchers should empirically determine the suitability of the MOTS-c mt-rnr1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MOTS-c mt-rnr1 product has the following accession number(s) (GI #756542780) (NCBI Accession #AJM13597.1) (Uniprot Accession #A0A0C5B5G6). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal Anti-MOTS-c antibody with the following immunoassay(s):
Data Tested
Western Blot (WB) (WB of MBS542112 with HepG2 cells.)
Immunohistochemistry (IHC) (Rat Skeletal Muscle- MOTS-c
Primary Antibody: MBS542112; 1:50 dilution in IHC Blocking Buffer.
DAB (brown) staining and Hematoxylin QS (blue) counterstain.
40X magnification on Leica DM4000. FFPE section.)
Mitochondria are functioning organelles that play a secondary role in signaling within the cell. While the details of mitochondrial signaling are not fully understood, the identification of an open reading frame in the mitochondrial DNA coding for humanin suggests that there are more signaling peptides coded by portions of the mtDNA. A short open reading frame within the 12S subunit of the mitochondrial ribosomal RNA codes for a 16 amino acid peptide called mitochondrial open reading frame of 12S rRNA-c, or MOTS-c. MOTS-c plays a role in insulin sensitivity and metabolic homeostasis (1). MOTS-c targets skeletal muscle tissue, and the mechanism of its action involves inhibition of the folate cycle. MOTS-c promotes the formation of an endogenous AMP analog called 5-aminoimidazole-4-carboxamide ribonucleotide (AICAR). AICAR is an activator of AMPK, suggesting that MOTS-c could be used in an insulin-regulation capacity in order to treat type 2 diabetes (2).
MOTS-c selective antibodies were generated against a peptide taken from the human protein. The MOTS-c-selective antibodies are affinity purified on an immobilized antigen based affinity matrix, the isolated antibodies were then stabilized in antibody stabilization buffer for long-term storage. The MOTS-c-selective antibodies are fully characterized for applications in western blotting and ELISA at the recommended dilutions. MyBioSource provides MOTS-c Western blot positive control samples in �ready-to-use� SDS-PAGE sample buffer. Antigenic blocking peptide for MOTS-c antibody is also available, please inquire before ordering. We can conjugate this antibody to fluorophores and other secondary enzymes as an additional service.