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anti-MS4A1 antibody product blog

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Posted on 2015-10-02 10:34:16 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; CD20; anti-MS4A1 antibody; MS4A1;
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The MS4A1 ms4a1 (Catalog #MBS176945) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-CD20 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD20 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC) Paraffin. Researchers should empirically determine the suitability of the MS4A1 ms4a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The MS4A1 ms4a1 product has the following accession number(s) (GI #115968) (NCBI Accession #P11836.1) (Uniprot Accession #P11836). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-CD20 Antibody with the following immunoassay(s):
Immunohistochemistry (IHC) (Figure 1. IHC analysis of CD20 using anti-CD20 antibody (MBS176945).
CD20 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD20 Antibody (MBS176945) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Immunohistochemistry (IHC) MS4A1.

Immunohistochemistry (IHC) (Figure 2. IHC analysis of CD20 using anti-CD20 antibody (MBS176945).
CD20 was detected in paraffin-embedded section of Human Tonsil Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-CD20 Antibody (MBS176945) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)
Immunohistochemistry (IHC) MS4A1.

Western Blot (WB) (Figure 3. Western blot analysis of CD20 using anti-CD20 antibody (MBS176945).
Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.
Lane 1: RAJI Whole Cell Lysate
Lane 2: CEM Whole Cell Lysate
After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CD20 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD20 at approximately 33KD. The expected band size for CD20 is at 33KD.)
Western Blot (WB) MS4A1.

Flow Cytometry (FC/FACS) (Figure 4. Flow Cytometry analysis of U937 cells using anti-CD20 antibody (MBS176945).
Overlay histogram showing U937 cells stained with MBS176945 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD20 Antibody (MBS176945,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight 488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
Flow Cytometry (FC/FACS) MS4A1.

Description: Rabbit IgG polyclonal antibody for B-lymphocyte antigen CD20(MS4A1) detection. Tested with WB, IHC-P in Human.
Background: CD20, also known as MS4A1, is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. It is mapped to 11q12.2. This gene encodes a member of the membrane-spanning 4A gene family. The function of CD20 is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that CD20 acts as a calcium channel in the cell membrane. What\'s more, this protein may be involved in the regulation of B-cell activation and proliferation.

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