|The MTBP mtbp (Catalog #MBS151250) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MTBP Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MTBP can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF).
MTBP antibody can be used for detection of MTBP by Western blot at 1 mug/mL. A 104 kDa band can be detected. Antibody can also be used for immunocytochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL. Researchers should empirically determine the suitability of the MTBP mtbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MTBP mtbp product has the following accession number(s) (GI #21630257) (NCBI Accession #NP_071328) (Uniprot Accession #Q96DY7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MTBP Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MTBP expression in K562 whole cell lysates with MTBP antibody at 1 μg /ml.)
Immunocytochemistry (ICC) (Immunocytochemistry of MTBP in K562 cells with MTBP antibody at 5 μg/mL.)
Immunofluorescence (IF) (Immunofluorescence of MTBP in K562 cells with MTBP antibody at 20 μg/mL.)
MTBP Antibody: The p53 tumor-suppressor gene integrates numerous signals that control cell life and death. Several novel molecules involved in p53 network, including Chk2, p53R2, p53AIP1, Noxa, PIDD, PID/MTA2 and MTBP, were recently discovered. The transcriptional activity of p53 is modulated by posttranslational regulations of the p53 protein including stabilization and acetylation. P53 transcriptionally activates MDM2 gene then the translated MDM2 protein binds to p53 and promotes the degradation of p53 leading to lowering the concentration of p53 protein. MDM2 inhibits both p53 mediated G1 arrest and apoptosis. A recently discovered protein termed MTBP was found to bind to MDM2 and to inhibit the modulation effect of MDM2 on p53. MTBP is expressed in a variety of normal tissues.
Immunogen: MTBP antibody was raised against a 18 amino acid peptide near the center of human MTBP.
Buffer: MTBP Antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MTBP are readily searchable from our website. Different antibodies against the same target such as MTBP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.