anti-MUC1 antibody product blog
Tags: Antibody; Polyclonal Antibody; MUC1; anti-MUC1 antibody;
The MUC1 muc1 (Catalog #MBS6000039) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MUC1, phosphorylated (T1224) (MUC1, PUM, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s MUC1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Immunofluorescence (IF), Gel Shift Assay (GS/EMSA).Suitable for use in Immunofluorescence, Dot Blot, ELISA
Dilution: ELISA: 1:1,000
Dot blot 1:500
Immunofluorescence: 1:10-50. Researchers should empirically determine the suitability of the MUC1 muc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MUC1 muc1 product has the following accession number(s) (GI #541680) (NCBI Accession #CAA56734.1) (Uniprot Accession #P15941). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MUC1, phosphorylated (T1224) (MUC1, PUM, Mucin-1, Breast carcinoma-associated antigen DF3, Carcinoma-associated mucin, Episialin, H23AG, Krebs von den Lungen-6, PEMT, Peanut-reactive urinary mucin, Polymorphic epithelial mucin, Tumor-associated epithelial with the following immunoassay(s):
Dot Blot (DB) (Dot blot analysis of anti-Phospho-MUC1-pT1224 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)
Immunofluorescence (IF) (Confocal immunofluorescent analysis of Phospho-MUC1-T1224 Antibody with Hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).)
MUC1 is a member of the mucin family and encodes a membrane bound, glycosylated phosphoprotein. The protein is anchored to the apical surface of many epithelia by a transmembrane domain, with the degree of glycosylation varying with cell type. It also includes a 20 aa variable number tandem repeat (VNTR) domain, with the number of repeats varying from 20 to 120 in different individuals. The protein serves a protective function by binding to pathogens and also functions in a cell signaling capacity. Overexpression, aberrant intracellular localization, and changes in glycosylation of this protein have been associated with carcinomas.
Immunogen: Phospho-MUC1-Pt1224 antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide between amino acids 1210-1240 surrounding T1224 of human MUC1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MUC1 are readily searchable from our website. Different antibodies against the same target such as MUC1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.