anti-MYH8 antibody product blog
Tags: Antibody; Polyclonal Antibody; Myh8; anti-MYH8 antibody;
The MYH8 myh8 (Catalog #MBS153376) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MYH8 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s MYH8 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Immunohistochemistry- Paraffin(IHC-P), Immunofluorescence (IF).MYH8 antibody can be used for detection of MYH8 by Western blot at 1 - 2 mug/ml. Antibody can also be used for immunohistochemistry starting at 5 mug/mL. For immunofluorescence start at 20 mug/mL.
Optimal dilutions for each application to be determined by the researcher.
Positive Control: 1) Cat. No. MBS151603 HeLa Cell Lysate. Researchers should empirically determine the suitability of the MYH8 myh8 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MYH8 myh8 product has the following accession number(s) (GI #153945790) (NCBI Accession #NP_002463) (Uniprot Accession #P13535). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MYH8 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of MYH8 in HeLa cell lysate with MYH8 antibody at (A) 0.5 and (B) 1 μg/ml.)
Immunohistochemistry (IHC) (Immunohistochemistry of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 5 μg/ml.)
Immunofluorescence (IF) (Immunofluorescence of MYH8 in mouse skeletal muscle tissue with MYH8 antibody at 20 μg/ml.)
Myosins are actin-based motor proteins that function in the generation of mechanical force in eukaryotic cells (1). MYH8 (myosin, heavy chain 8, skeletal muscle, perinatal) is a member of the class II or conventional myosin heavy chains, and functions in skeletal muscle contraction (2,3). This gene is predominantly expressed in fetal skeletal muscle. MYH8 is regulated by phosphorylation via myosin light chain kinase (MLCK) and by intracellular Ca2+ concentrations (3). A mutation in this gene results in trismus-pseudocamptodactyly syndrome (4).
Conjugate: Unconjugated
Immunogen: MYH8 antibody was raised against a 19 amino acid peptide near the amino terminus of human MYH8.
Buffer: MYH8 antibody is supplied in PBS containing 0.02% sodium azide. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing MYH8 are readily searchable from our website. Different antibodies against the same target such as MYH8 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.