anti-MYLK antibody product blog
Tags: Antibody; Polyclonal Antibody; MYLK; anti-MYLK antibody; MLCK;
The MYLK mylk (Catalog #MBS9215191) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The MLCK Antibody (N-term) reacts with Human, mouse (Predicted Reactivity: Rabbit) and may cross-react with other species as described in the data sheet. MyBioSource\'s MLCK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC-P).WB~~1:1000
IHC~P~~1:50~100. Researchers should empirically determine the suitability of the MYLK mylk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The MYLK mylk product has the following accession number(s) (GI #116008192) (NCBI Accession #NP_444253.3) (Uniprot Accession #Q15746). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MLCK Antibody (N-term) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). MBS9215191 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB) (Western blot analysis of lysate from mouse bladder tissue lysate, using MLCKlong Antibody (M1). MBS9215191 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 35ug.)
Western Blot (WB) (MLCK Antibody (M1) western blot analysis in SK-BR-3 cell line lysates (35ug/lane).This demonstrates the MLCK antibody detected the MLCK protein (arrow).)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Western Blot (WB) (Western blot analysis of anti-MLCK-long Pab in mouse brain tissue lysate. MLCK-long (Arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
MLCK, a member of the Ser/Thr protein kinase family, is a calcium/calmodulin-dependent enzyme responsible for smooth muscle contraction via phosphorylation of a specific serine in the N-terminus of myosin light chains (MLC), an event that facilitates myosin interaction with actin filaments. It is a central determinant in the development of vascular permeability and tissue edema formation. In the nervous system it has been shown to control the growth initiation of astrocytic processes in culture and to participate in transmitter release at synapses formed between cultured sympathetic ganglion cells. MLCK acts as a critical participant in signaling sequences that result in fibroblast apoptosis. Smooth muscle and non-muscle isozymes are expressed in a wide variety of adult and fetal tissues and in cultured endothelium with qualitative expression appearing to be neither tissue- nor development-specific. Non-muscle isoform 2 is the dominant splice variant expressed in various tissues. The Telokin isoform, which binds calmodulin, has been found in a wide variety of adult and fetal tissues. MLCK is probably down-regulated by phosphorylation. The protein contains 1 fibronectin type III domain and 9 immunoglobulin-like C2-type domains.