anti-NAA10 antibody product blog
Tags: Antibody; Polyclonal Antibody; NAA10; anti-Naa10 antibody;
The NAA10 naa10 (Catalog #MBS9126002) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s NAA10 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).Recommended Dilutions
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:100. Researchers should empirically determine the suitability of the NAA10 naa10 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NAA10 naa10 product has the following accession number(s) (GI #728880) (NCBI Accession #P41227.1) (Uniprot Accession #P41227). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NAA10 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NAA10 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat lung using NAA10 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using NAA10 antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using NAA10 antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of U2OS cells using NAA10 antibody. Blue: DAPI for nuclear staining.)
N-alpha-acetylation is among the most common post-translational protein modifications in eukaryotic cells. This process involves the transfer of an acetyl group from acetyl-coenzyme A to the alpha-amino group on a nascent polypeptide and is essential for normal cell function. This gene encodes an N-terminal acetyltransferase that functions as the catalytic subunit of the major amino-terminal acetyltransferase A complex. Mutations in this gene are the cause of Ogden syndrome. Alternate splicing results in multiple transcript variants.
Species Reactivity/Cross-Reactivity: Human, Mouse, Rat. Immunogen: Recombinant fusion proteincontaining a sequence corresponding to amino acids 161-235 of human NAA10
Calculated Molecular Weight: 26kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NAA10 are readily searchable from our website. Different antibodies against the same target such as NAA10 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.