anti-NARS antibody product blog
Tags: Antibody; NARS; anti-NARS antibody; Polyclonal Antibody; Asparaginyl tRNA Synthetase;
The NARS nars (Catalog #MBS2033934) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Asparaginyl tRNA Synthetase (NARS) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Asparaginyl tRNA Synthetase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA).Western blotting: 1:50-400
Immunohistochemistry in formalin fixed frozen section: 1:50-500
Enzyme-linked Immunosorbent Assay: 1:100-1:5000. Researchers should empirically determine the suitability of the NARS nars for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NARS nars product has the following accession number(s) (GI #4758762) (NCBI Accession #NP_004530.1) (Uniprot Accession #O43776). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Asparaginyl tRNA Synthetase (NARS) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Human K562 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NARS Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB) (Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin- embedded kidney tissue))
Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Human Stomach Tissue))
Knockout Validation (Knockout Validation: Lane 1: Wild-type K562 cell lysate; Lane 2: NARS knockout K562 cell lysate; Predicted MW: 20,63kd Observed MW: 65kd Primary Ab: 1ug/ml Rabbit Anti-Human NARS Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Fragment: NARS (Met1~Arg330)
Conjugate: No Conjugate
Cross Reactivity: Human. Immunoglobulin Type: IgG
Quality Control: Content: The quality control contains recombinant NARS (Met1~Arg330) disposed in loading buffer.
Usage: 10uL per well when 3,3\'-Diaminobenzidine(DAB) as the substrate.
5uL per well when used in enhanced chemilumescent (ECL).
Note: The quality control is specifically manufactured as the positive control. Not used for other purposes.
Loading Buffer: 100mM Tris(pH8.8), 2% SDS, 200mM NaCl, 50% glycerol,BPB 0.01%, NaN3 0.02%.
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2069344. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NARS are readily searchable from our website. Different antibodies against the same target such as NARS may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.