anti-NCK1 antibody product blog
Tags: Antibody; Polyclonal Antibody; NCK1; anti-NCK1 antibody;
The NCK1 nck1 (Catalog #MBS9402221) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NCK1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s NCK1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).Western blotting: 1:200-1:1000
Immunohistochemistry: 1:50-1:200. Researchers should empirically determine the suitability of the NCK1 nck1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NCK1 nck1 product has the following accession number(s) (GI #299829184) (NCBI Accession #NP_001177725.1) (Uniprot Accession #P16333). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NCK1 Antibody with the following immunoassay(s):
Testing Data (Gel: 10%SDS-PAGE Lysates (from left to right): Jurkat and K562 cell Amount of lysate: 40ug per lane Primary antibody: 1/600 dilution Secondary antibody dilution: 1/8000 Exposure time: 1 minute)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Human lung cancer tissue using at dilution 1/30.)
The protein encoded by this gene is one of the signaling and transforming proteins containing Src homology 2 and 3 (SH2 and SH3) domains. It is located in the cytoplasm and is an adaptor protein involved in transducing signals from receptor tyrosine kinases to downstream signal recipients such as RAS. Alternatively spliced transcript variants encoding different isoforms have been found.
Immunogen Type: Recombinant Protein
Immunogen Description: Fusion protein corresponding to residues near the C terminal of human NCK adaptor protein 1. Target Name: NCK1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NCK1 are readily searchable from our website. Different antibodies against the same target such as NCK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Atrophy, Carcinoma, Hepatocellular, Carcinoma, Squamous Cell, Kidney Diseases, Liver Diseases, Lung Neoplasms, Neoplasm Metastasis, Nerve Degeneration, Pancreatic Neoplasms, Proteinuria are some of the diseases may be linked to NCK1 Antibody. Eye, Lung, Pancreas tissues are correlated with this protein. NCK1 also interacts with the following gene(s): CBL, EGFR, PAK1, PDGFRB, WASL. The following patways have been known to be associated with this gene.