|The NCSTN ncstn (Catalog #MBS176992) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-NCSTN antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NCSTN can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Researchers should empirically determine the suitability of the NCSTN ncstn for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NCSTN ncstn product has the following accession number(s) (GI #12231037) (NCBI Accession #Q92542.2) (Uniprot Accession #Q92542). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-NCSTN antibody with the following immunoassay(s):
Western Blot (WB) (Anti-NCSTN antibody, MBS176992, All Western blotting
All lanes: Anti-NCSTN(MBS176992) at 0.5ug/ml
Lane 1: Rat Brain Tissue Lysate at 40ug
Predicted bind size: 78KD
Observed bind size: 78KD )
Description: Rabbit IgG polyclonal antibody for Nicastrin(NCSTN) detection. Tested with WB in Human, Mouse, Rat.
Background: Nicastrin, also known as NCSTN, is a protein that is a part of the gamma secretase protein complex. It is mapped to 1q23.2. This gene is not catalytically active, but instead promotes the maturation and proper trafficking of the other proteins in the gamma secretase protein complex, all of which undergo significant post-translational modification before becoming active in the cell. NCSTN encodes a Type I transmembrane glycoprotein that is an integral component of the multimeric gamma-secretase complex. The encoded protein cleaves integral membrane proteins, including Notch receptors and beta-amyloid precursor protein, and may be a stabilizing cofactor required for gamma-secretase complex assembly. The cleavage of beta-amyloid precursor protein yields amyloid beta peptide, the main component of the neuritic plaque and the hallmark lesion in the brains of patients with Alzheimer\'s disease. Nicastrin has also been identified as a regulator of neprilysin, an enzyme involved in the degradation of amyloid beta fragment.
Immunogen: A synthetic peptide corresponding to a sequence at the N-terminus of human Nicastrin (72-88aa VIHVVEKEEDLQWVLTD), different from the related mouse sequence by one amino acid, and from the related rat sequence by two amino acids. Contents: Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg Thimerosal, 0.05mg NaN3.
Reconstitution: Add 0.2ml of distilled water will yield a concentration of 500ug/ml. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NCSTN are readily searchable from our website. Different antibodies against the same target such as NCSTN may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.