anti-Neprilysin-Like Protease, alpha antibody product blog
Tags: Antibody; Polyclonal Antibody; Neprilysin-Like Protease, alpha; anti-Neprilysin-Like Protease, alpha antibody;
The Neprilysin-Like Protease, alpha n/a (Catalog #MBS618625) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Neprilysin-Like Protease, alpha (NEPL-a) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Neprilysin-Like Protease, alpha can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB).Dilution: ELISA: 1:5000-1:50,000 using 50-100ng of control peptide (N2030-34) per well.
Western Blot: 1:1000-1:5000 using ECL. Researchers should empirically determine the suitability of the Neprilysin-Like Protease, alpha n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The amyloid beta peptide (Ab) of 39-43aa is constitutively produced in brain upon proteolysis of the b-amyloid precursor protein (APP) and exists as fragments of 40, 42 and 43aa (Ab40, Ab42, Ab43). In young and healthy humans, Ab is rapidly catabolized before it can be deposited in the brain. Upon aging or the onset of familial Alzheimer\'s Disease however, alterations in either synthesis or degradation/ clearance of Ab may contribute to amyloid depositions in the brain. Ab is susceptible to a number of in vivo and in vitro proteases like cathepsin-D and M-13 metalloproteases. The M-13 family comprises several zinc-dependent metalloproteases like DINE, PHEX, KELL, ECE, XCE, neprilysin (NEP) and neprilysin-like proteases (NEPLs). The NEPLs (NEPL-alpha, NEPL-beta, NEPL-gamma) arise from the alternative splicing of a single NEPL gene and are zinc dependent metalloproteases with ~54 % homology to NEP. NEPL-an is a type II trans-membrane metallopeptidase (~100kD non-glycosylated and 120kD glycosylated) containing a highly glycosylated polypeptide chain of 742aa with a HEXXH zinc-binding consensus, cytoplasmic tail and a large extracellular catalytic domain. NPL-a lacks a 23aa sequence present in N-terminal of NEPL-b. In contrast to NEP and NEPL-b which exist in membrane-bound as well as soluble forms, NEPL-an is expressed as membrane-bound form only. In comparison to NEP, the NEPL-a has lower affinity and/or smaller Vmax for Ab and cannot proteolyze cell secreted Ab40 or Ab42 in vivo. Unlike NEP, NEPL-an is not a major in vivo peptidase for degrading endogenous Ab however, it might degrade Ab in Alzheimer patients where Ab is accumulated in excess and an Ab-degradative pathway, alternative to NEP, exists.
Immunogen: Synthetic peptide corresponding to 17aa from mouse Neprilysin-Like Protease, alpha (NEPL-a) conjugated to KLH (NO038811.1). Epitope: ~N-terminal, Cytoplasmic domain. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing Neprilysin-Like Protease, alpha are readily searchable from our website. Different antibodies against the same target such as Neprilysin-Like Protease, alpha may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.