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anti-NES antibody product blog

Posted on 2015-12-19 08:49:41 by mybiosource_staff
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Tags: Antibody; NES; Polyclonal Antibody; anti-NES antibody;
The NES prf1 (Catalog #MBS859018) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NES Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NES can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:500-1:2000
IHC: 1:50-1:200. Researchers should empirically determine the suitability of the NES prf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NES prf1 product has the following accession number(s) (GI #133908621) (NCBI Accession #NP_001076585.1) (Uniprot Accession #P14222). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NES Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various celllines, using PRF1 antibody.)
Western Blot (WB) NES.

Granzymes are a family of serine proteases expressed by cytotoxic T lymphocytes and natural killer (NK) cells and are key components of immune responses to pathogens and transformed cells (1). Granzymes are synthesized as zymogens and are processed into mature enzymes by cleavage of a leader sequence. They are released by exocytosis in lysosome-like granules containing perforin, a membrane pore-forming protein. Granzyme B has the strongest apoptotic activity of all the granzymes as a result of its caspase-like ability to cleave substrates at aspartic acid residues thereby activating procaspases directly and cleaving downstream caspase substrates (2, 3). Perforin is a pore-forming protein that facilitates the entry of cytotoxic serine proteases, such as granzymes, into target cells (4). Perforin is primarily expressed in cytotoxic T lymphocytes and NK cells.

Immunogen: Recombinant protein of human PRF1
Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NES are readily searchable from our website. Different antibodies against the same target such as NES may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Carcinoma, Cardiovascular Diseases, Drug Toxicity, Immune System Diseases, Inflammation, Liver Diseases, Lymphoma, Non-Hodgkin, Necrosis, Neoplasms, Neoplasms, Experimental are some of the diseases may be linked to NES Antibody. NES also interacts with the following gene(s): CD247, CD3D, CD3E, CD86, EOMES, GZMB, IL2, IL2RA, IL2RB.
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