anti-NF1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NF1 antibody; NF1; Neurofibromin / NF1;
The NF1 nf1 (Catalog #MBS244810) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human Neurofibromin / NF1 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Neurofibromin / NF1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).ELISA (1:20000), IF (1:100 - 1:500), IHC-P (1:100), WB (1:500 - 1:1000). Researchers should empirically determine the suitability of the NF1 nf1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NF1 nf1 product has the following accession number(s) (GI #4557793) (NCBI Accession #NP_000258.1) (Uniprot Accession #P21359). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Human Neurofibromin / NF1 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-NF1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunohistochemistry (IHC) (Anti-NF1 antibody IHC of human tonsil. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.)
Immunofluorescence (IF) (Immunofluorescence of HepG2 cells, using NF1 Antibody. The picture on the right is treated with the synthesized peptide.)
Western Blot (WB) (Western blot of extracts from HepG2 cells, using NF1 Antibody. The lane on the right is treated with the synthesized peptide.)
Mutations linked to neurofibromatosis type 1 led to the identification of NF1. NF1 encodes the protein neurofibromin, which appears to be a negative regulator of the ras signal transduction pathway. In addition to type 1 neurofibromatosis, mutations in NF1 can also lead to juvenile myelomonocytic leukemia. Alternatively spliced NF1 mRNA transcripts have been isolated, although their functions, if any, remain unclear.
Target Species: Human
Immunogen Description: Synthetic peptide from human NF1 (aa1551-1600). Immunogen: Neurofibromin / NF1 antibody was raised against synthetic peptide from human NF1 (aa1551-1600).
Antigen Modification: aa1551-1600. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NF1 are readily searchable from our website. Different antibodies against the same target such as NF1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.