anti-NGAL antibody product blog
Tags: Antibody; Polyclonal Antibody; NGAL ; anti-NGAL antibody;
The NGAL lcn2 (Catalog #MBS715575) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit anti-human NGAL polyclonal Antibody reacts with Human. Other species are not tested. Please decide the specificity by homology. and may cross-react with other species as described in the data sheet. MyBioSource\'s NGAL can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB). Researchers should empirically determine the suitability of the NGAL lcn2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The NGAL lcn2 product has the following accession number(s) (GI #38455402) (NCBI Accession #NP_005555.2) (Uniprot Accession #P80188). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit anti-human NGAL polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot
All lanes: NGAL Antibody at 2ug/ml+positive serum
Lane 1: positive serum at 1: 10
Lane 2: positive serum at 1: 100
Lane 3: positive serum at 1: 500
Secondary
Goat polyclonal to Rabbit IgG at 1/15000 dilution
Predicted band size: 22 kDa
Observed band size: 25 kDa
)
Iron-trafficking protein involved in multiple processes such as apoptosis, innate immunity and renal development. Binds iron through association with 2,5-dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin, and delivers or removes iron from the cell, depending on the context. Iron-bound form (holo-24p3) is internalized following binding to the SLC22A17 (24p3R) receptor, leading to release of iron and subsequent increase of intracellular iron concentration. In contrast, association of the iron-free form (apo-24p3) with the SLC22A17 (24p3R) receptor is followed by association with an intracellular siderophore, iron chelation and iron transfer to the extracellular medium, thereby reducing intracellular iron concentration. Involved in apoptosis due to interleukin-3 (IL3) deprivation: iron-loaded form increases intracellular iron concentration without promoting apoptosis, while iron-free form decreases intracellular iron levels, inducing expression of the proapoptotic protein BCL2L11/BIM, resulting in apoptosis. Involved in innate immunity, possibly by sequestrating iron, leading to limit bacterial growth.
Storage Buffer: Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
Conjugate: Non-conjugated. Immunogen: Recombinant human NGAL protein
Santa Cruz Alternative: Potential replacement for Santa Cruz Biotechnology antibody catalog# sc-18695 / sc-50350. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NGAL are readily searchable from our website. Different antibodies against the same target such as NGAL may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.