anti-NLK antibody product blog
Tags: Antibody; Polyclonal Antibody; Nlk; anti-nlk antibody;
The NLK nlk (Catalog #MBS127638) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NLK Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NLK can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the NLK nlk for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NLK nlk product has the following accession number(s) (GI #262527551) (NCBI Accession #Q9UBE8.2) (Uniprot Accession #Q9UBE8). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NLK Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NLK antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat spinal cord using NLK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded rat brain using NLK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human lung using NLK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded human stomach using NLK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse lung using NLK antibody at dilution of 1:100 (40x lens).)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse spleen using NLK antibody at dilution of 1:100 (40x lens).)
The activation of signal transduction pathways by growth factors, hormones and neurotransmitters is mediated through two closely related MAP kinases, p44 and p42, designated extracellular-signal related kinase 1 (ERK 1) and ERK 2, respectively. ERK proteins are regulated by dual phosphorylation at specific tyrosine and threonine sites mapping within a characteristic Thr-Glu-Tyr motif. Phosphorylation at both Thr-183 and Tyr-185 is required for full enzymatic activation. In response to activation, MAP kinases phosphorylate downstream components on serine and threonine (5,6). Nlk, or nemo-like kinase, is a murine homolog of the Drosophila nemo (nmo) gene. Nlk and Nmo have sequence homology to both the ERK MAP kinases and the cyclin dependent kinases. Nlk is a nuclear protein with the ability to autophosphorylate.
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human NLK
Calculated Molecular Weight: 58kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NLK are readily searchable from our website. Different antibodies against the same target such as NLK may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.