anti-NLRC4 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NLRC4 antibody; NLRC4;
The NLRC4 nlrc4 (Catalog #MBS9126899) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NLRC4 reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NLRC4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200. Researchers should empirically determine the suitability of the NLRC4 nlrc4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NLRC4 nlrc4 product has the following accession number(s) (GI #20138032) (NCBI Accession #Q9NPP4.2) (Uniprot Accession #Q9NPP4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NLRC4 with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NLRC4 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using NLRC4 antibody at dilution of 1:100 (40x lens).)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cells using NLRC4 antibody. Blue: DAPI for nuclear staining.)
This gene encodes a member of the caspase recruitment domain-containing NLR family. Family members play essential roles in innate immune response to a wide range of pathogenic organisms, tissue damage and other cellular stresses. Mutations in this gene result in autoinflammation with infantile enterocolitis. Alternative splicing results in multiple transcript variants.
Species: Human
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-250 of human NLRC4 (NP_067032.3). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NLRC4 are readily searchable from our website. Different antibodies against the same target such as NLRC4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Bladder, Blood, Bone Marrow, Brain, Kidney, Liver, Testis, Thymus, Uterus tissues are correlated with this protein. Disease Models, Animal, Enterocolitis, Immune System Diseases, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Nervous System Diseases, Pneumonia, Weight Loss are some of the diseases may be linked to NLRC4.