|The NME1 nme1 (Catalog #MBS9409413) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NME1 Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NME1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF).
Western blotting: 1:500 - 1:2000
Immunohistochemistry: 1:50 - 1:200
Immunofluorescence: 1:50 - 1:200. Researchers should empirically determine the suitability of the NME1 nme1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NME1 nme1 product has the following accession number(s) (GI #4557797) (NCBI Accession #NP_000260.1) (Uniprot Accession #P15531). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NME1 Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NME1/NDKA antibody.)
Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded Pancreas cancer using NME1 Antibody.)
Immunofluorescence (IF) (Immunofluorescence analysis of A549 cell using NME1 antibody. Blue: DAPI for nuclear staining.)
The NDK/NME/NM23 kinase family (encoded by the NME gene family) consists of at least eight distinct proteins that exhibit different cellular localization (1). Members of this group inhibit metastasis in a variety of tumor cell types (2). All NDK/NME/NM23 proteins possess nucleoside diphosphatase kinase (NDK) activity and catalyze the phosphorylation of nucleoside diphosphate to the corresponding nucleoside triphosphate to regulate a diverse array of cellular events (3). At least four classes of NDK biochemical activities have been described, including protein-protein interactions (4-6), regulation of GTP-binding protein function (7-9), DNA-associated activities (10,11), and histidine-dependent protein phosphotransferase activity (12). NDK/NME proteins participate in the regulation of a broad spectrum of cellular responses, including development, differentiation, proliferation, endocytosis, and apoptosis (13). Because of its role in metastasis suppression and oncogenesis, NDKA (NME1/NM23-H1) has been widely studied (14). NDKA (NM23-H1) and NDKB (NM23-H2) are encoded by adjacent NME1 and NME2 genes and share 90% sequence identity. Two serine residues (Ser122 and Ser144) on NDKA/NM23-H1 can be phosphorylated by AMPKa1, but only phosphorylation at Ser122 determines whether NDKA channels ATP to AMPKa1. This regulates AMPKa1 activity towards ACC1, an important regulator of fatty acid metabolism (15). Mutation of NDKB/NM23-H2 at Ser122 (S122P) in melanoma cells results in altered phosphoryl transfer activity (16).
Immunogen Type: Peptide
Immunogen Description: A synthetic peptide of human NME1. Target Name: NME1. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NME1 are readily searchable from our website. Different antibodies against the same target such as NME1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.