anti-NNE antibody product blog
Tags: Antibody; Polyclonal Antibody; Enolase; NNE; anti-NNE antibody;
The NNE eno1 (Catalog #MBS2026649) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Enolase, Non Neuronal (NNE) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s Enolase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA).Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
. Researchers should empirically determine the suitability of the NNE eno1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NNE eno1 product has the following accession number(s) (GI #319996655) (NCBI Accession #NP_001188412.1) (Uniprot Accession #P06733). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and T7-tag and its sequence is listed below.
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MSILKIHARE IFDSRGNPTV EVDLFTSKGL FRAAVPSGAS TGIYEALELR DNDKTRYMGK GVSKAVEHIN KTIAPALVSK KLNVTEQEKI DKLMIEMDGT ENKSKFGANA ILGVSLAVCK AGAVEKGVPL YRHIADLAGN SEVILPVPAF NVINGGSHAG NKLAMQEFMI LPVGAANFRE AMRIGAEVYH NLKNVIKEKY GKDATNVGDE GGFAPNILEN KEGLELLKTA IGKAGYTDKV VIGMDVAASE FFRSGKYDLD FKSPDDPSRY ISPDQLADLY KSFIKDYPVV SIEDPFDQDD WGAWQKFTAS AGIQVVGDDL TVTNPKRIAK AVNEKSCNCL LLKVNQIGSV TESLQACKLA QANGWGVMVS HRSGETEDTF IADLVVGLCT GQIKTGAPCR SERLAKYNQL LRIEEELGSK AKFAGRNFRN PLAK.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Enolase, Non Neuronal (NNE) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Human Lung lysate; Primary Ab: 1ug/ml Rabbit Anti-Human NNE Antibody Second Ab: 0.2ug/mL�HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB) (Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC) (DAB staining on fromalin fixed paraffin-embedded Stomach tissue))
Fragment: NNE (Met1~Lys434)
Organism Species: Homo sapiens (Human)
Cross Reactivity: Human, Mouse. Conjugate: No Conjugate
Immunogen: Recombinant NNE (Met1~Lys434) expressed in E Coli.
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2055307. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NNE are readily searchable from our website. Different antibodies against the same target such as NNE may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.