anti-NOD1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NOD1 antibody; NOD1; CARD4;
The NOD1 nod1 (Catalog #MBS613440) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The CARD4 (Caspase Recruitment Domain, Nod1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CARD4 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot.
Dilution: Western Blot: 1:1000
Optimal dilution determined by the researcher. Researchers should empirically determine the suitability of the NOD1 nod1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOD1 nod1 product has the following accession number(s) (GI #4760400) (Uniprot Accession #Q9Y239). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CARD4 (Caspase Recruitment Domain, Nod1) with the following immunoassay(s):
Western Blot (WB) (Figure: Detection of MBS613440 in HEK 293T transfected cells by the MBS613440 Method: 10µg per lane of total cell extracts from mock transfected HEK 293T cells (lane 1) or HEK 293T cells transfected with a plasmid coding for MBS613440 (lane 2) were run on a SDS-PAGE gel and analyzed by Western blot. After transfer to nitrocellulose, the membrane was probed with MBS613440 at a 1:1’000 dilution. After 1 hour of incubation at RT, the membrane was extensively washed and then incubated with goat anti-rabbit horseradish peroxidase (1:5’000 dilution; Jackson, for ECL detection.)
Immunogen: Synthetic peptide corresponding to aa 2-31 (E2EQGHSEMEIIP SESHPHIQLLKSNRELLV31) of human CARD4. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOD1 are readily searchable from our website. Different antibodies against the same target such as NOD1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Eye, Intestine, Kidney, Liver, Lung, Mouth, Spleen, Vascular tissues are correlated with this protein. The following patways have been known to be associated with this gene. NOD1 also interacts with the following gene(s): CARD6, CASP1, CASP8, CASP9, NLRC4, NLRP1, RIPK2. Drug-Induced Liver Injury, Lung Diseases, Interstitial, Translocation, Genetic are some of the diseases may be linked to CARD4 (Caspase Recruitment Domain, Nod1).