anti-NOG antibody product blog
Tags: Antibody; Noggin; NOG; Polyclonal Antibody; anti-NOG antibody;
The NOG nog (Catalog #MBS2027819) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Noggin (NOG) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Noggin can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Paraffin, ELISA (EIA).Western blotting: 0.2-2ug/mL;1:250-2500
Immunohistochemistry: 5-20ug/mL;1:25-100
Immunocytochemistry: 5-20ug/mL;1:25-100
Optimal working dilutions must be determined by end user.
. Researchers should empirically determine the suitability of the NOG nog for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOG nog product has the following accession number(s) (GI #15214085) (NCBI Accession #Q62809.1) (Uniprot Accession #Q62809). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
Antigen: The target protein is fused with two N-terminal Tags, His-tag and MBP-tag and its sequence is listed below.
MKIEEGKLVI WINGDKGYNG LAEVGKKFEK DTGIKVTVEH PDKLEEKFPQ VAATGDGPDI IFWAHDRFGG YAQSGLLAEI TPDKAFQDKL YPFTWDAVRY NGKLIAYPIA VEALSLIYNK DLLPNPPKTW EEIPALDKEL KAKGKSALMF NLQEPYFTWP LIAADGGYAF KYENGKYDIK DVGVDNAGAK AGLTFLVDLI KNKHMNADTD YSIAEAAFNK GETAMTINGP WAWSNIDTSK VNYGVTVLPT FKGQPSKPFV GVLSAGINAA SPNKELAKEF LENYLLTDEG LEAVNKDKPL GAVALKSYEE ELAKDPRIAA TMENAQKGEI MPNIPQMSAF WYAVRTAVIN AASGRQTVDE ALKDAQTGST SGSGHHHHHH SAGLVPRGST AIGMKETAAA KFERQHMDSP DLGTLEVLFQ GPLGSEF-MP S EIKGLEFSEG LAQGKKQRLS KKLRRKLQMW LWSQTFCPVL YAWNDLGSRF WARYVKVGSC FSKRSCSVPE GMVCKPSKSV HLTVLRWRCQ RRGGQRCGWI PIQYPIISEC KCSC.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Noggin (NOG) with the following immunoassay(s):
Western Blot (WB) (Western Blot: Sample: Recombinant protein.)
Immunohistochemistry (IHC) (DABstainingonIHC-P.Samples:RatTissue))
Fragment: NOG (Met28~Cys144)
Organism Species: Rattus norvegicus (Rat)
Cross Reactivity: Rat. Conjugate: No Conjugate
Immunogen: Recombinant NOG (Met28~Cys144) expressed in E Coli.
Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2063483. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOG are readily searchable from our website. Different antibodies against the same target such as NOG may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Embryonic Tissue, Heart tissues are correlated with this protein.