Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
1.855.MyBioSource
Fax 1.858.633.0166    Email sales@mybiosource.com 
tel/fax
menu1 Home Products Ordering Company Help menu7
bottom
   MyBioSource  rightarrow Polyclonal Antibody Blog  rightarrow anti-NOS3 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
separator
 • anti-GK3P antibody blog
 • anti-ANXA1 antibody blog
 • anti-MERTK antibody blog
 • anti-C1QTNF6 antibody blog
 • anti-ZBTB42 antibody blog
 • anti-ABI3BP antibody blog
 • anti-CD2 antibody blog
 • anti-UGGT1 antibody blog
 • anti-ASMT antibody blog
 • anti-DEF8 antibody blog
 • anti-RELB antibody blog
 • anti-FAK antibody blog
 • anti-FSCN antibody blog
 • anti-APLNR antibody blog
 • anti-RPL28 antibody blog
 • anti-tankyrase antibody blog
 • anti-CRHR1 antibody blog
 • anti-PCBP4 antibody blog
 • anti-TERF2 antibody blog
 • anti-GSK3a antibody blog
separator
Blog Categories separator
separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
separator
Our Best Sellers moreseparator
separator
 • SCIN Antibody
 • alpha-Smooth muscle actin (alpha-SMA) ELISA Kit
 • alpha1-AGP (Alpha-1-Acid Glycoprotein) CLIA Kit
 • M-lycotoxin-Ls3b (M-LCTX-Ls3b) Recombinant Protein
 • Carboxyterminal propeptide of type I  procollagen, PI CP (PI... ELISA Kit
 • Fibroblast Growth Factor 7 (FGF7) Recombinant Protein
 • N-Terminal Pro-Brain Natriuretic Peptide (NT-ProBNP) ELISA Kit
 • Thyrotropin Releasing Hormone (TRH) ELISA Kit
 • ring finger and FYVE-like domain containing 1 (RFFL) ELISA Kit
 • Duffy Blood Group Chemokine Receptor (DARC) Recombinant Protein
 • Collagen Type IV (Col IV) ELISA Kit
 • Cluster of Differentiation 3e (CD3e) ELISA Kit
 • Actin, alpha cardiac muscle 1 (ACTC1) ELISA Kit
 • Lipid Peroxide (LPO) ELISA Kit
 downarrow more ...
separator

anti-NOS3 antibody product blog

separator
Posted on 2015-12-21 15:58:50 by mybiosource_staff
Share it on ...     
separator
separator
Tags: Antibody; Polyclonal Antibody; anti-NOS3 antibody; NOS3;
separator
The NOS3 nos3 (Catalog #MBS126917) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NOS3 Polyclonal Antibody reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s NOS3 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200. Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NOS3 nos3 product has the following accession number(s) (GI #266648) (NCBI Accession #P29474.3) (Uniprot Accession #P29474). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NOS3 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using NOS3 antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 30s.)
Western Blot (WB) NOS3.

Endothelial nitric-oxide synthase (eNOS) is an important enzyme in the cardiovascular system. It catalyzes the production of nitric oxide (NO), a key regulator of blood pressure, vascular remodeling, and angiogenesis (1,2). The activity of eNOS is regulated by phosphorylation at multiple sites. The two most thoroughly studied sites are the activation site Ser1177 and the inhibitory site Thr495 (3). Several protein kinases including Akt/PKB, PKA, and AMPK activate eNOS by phosphorylating Ser1177 in response to various stimuli (4,5). In contrast, bradykinin and H2O2 activate eNOS activity by promoting both Ser1177 phosphorylation and Thr495 dephosphorylation (6,7). eNOS is activated by VEGF, and this activation is associated with dephsophorylation of eNOS at serine 113. Cyclosporin A blocks this dephosphorylation of eNOS upon VEGF stimulation (8).

Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human NOS3
Calculated Molecular Weight: 133kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOS3 are readily searchable from our website. Different antibodies against the same target such as NOS3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone, Brain, Embryonic Tissue, Heart, Kidney, Liver, Lung, Skin, Vascular tissues are correlated with this protein.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions