anti-NOS3 antibody product blog
Tags: Antibody; Polyclonal Antibody; NOS3 / eNOS; anti-NOS3 antibody; NOS3;
The NOS3 nos3 (Catalog #MBS247351) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Rabbit Polyclonal (IgG) to Human NOS3 / eNOS reacts with Gorilla, Human, Monkey, RabbitPredicted Reactivity: Mouse, Rat, Bovine, Guinea pig, Hamster (at least 90% immunogen sequence identity) and may cross-react with other species as described in the data sheet. MyBioSource\'s NOS3 / eNOS can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Western Blot (WB).
IHC-P (5 ug/ml), WB
Usage: Suitable for use in Western Blot and IHC-P. Western Blot Analysis: 0.5-2 ug/ml of this lot detected eNOS/NOS III in RIPA lysates from HUVEC cells. Researchers should empirically determine the suitability of the NOS3 nos3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NOS3 nos3 product has the following accession number(s) (GI #40254422) (NCBI Accession #NP_000594.2) (Uniprot Accession #P29474). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Rabbit Polyclonal (IgG) to Human NOS3 / eNOS with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-ENOS antibody IHC of human placenta. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) (Anti-ENOS antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
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Target Species: Human
Immunogen Description: KLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Immunogen Type: Synthetic peptide - KLH conjugated
Immunogen: NOS3 / eNOS antibody was raised against kLH-conjugated, synthetic peptide corresponding to aa1181-1194 (C-QERQLRGAVPWAFD) of human eNOS/NOS III. The immunizing sequence has 10/11 identical amino acids in mouse, guinea pig, bovine, porcine, rat and canine. Percent identity by BLAST analysis: Human, Gorilla, Monkey, Elephant, Rabbit (100%); Mouse, Rat, Hamster, Panda, Bovine, Guinea pig (93%); Dog, Bat, Pig (86%).
Antigen Modification: aa1181-1194. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NOS3 are readily searchable from our website. Different antibodies against the same target such as NOS3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.