anti-NPHS1 antibody product blog
Tags: Antibody; Polyclonal Antibody; anti-NPHS1 antibody; NPHS1;
The NPHS1 nphs1 (Catalog #MBS127602) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NPHS1 Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s NPHS1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC).WB: 1:500 - 1:2000
IHC: 1:50 - 1:100. Researchers should empirically determine the suitability of the NPHS1 nphs1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NPHS1 nphs1 product has the following accession number(s) (GI #20177993) (NCBI Accession #O60500.1) (Uniprot Accession #O60500). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NPHS1 Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of mouse kidney, using NPHS1 antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunohistochemistry (IHC) (Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (40x lens).)
This gene encodes a member of the immunoglobulin family of cell adhesion molecules that functions in the glomerular filtration barrier in the kidney. The gene is primarily expressed in renal tissues, and the protein is a type-1 transmembrane protein found at the slit diaphragm of glomerular podocytes. The slit diaphragm is thought to function as an ultrafilter to exclude albumin and other plasma macromolecules in the formation of urine. Mutations in this gene result in Finnish-type congenital nephrosis 1, characterized by severe proteinuria and loss of the slit diaphragm and foot processes. In Western blots, nephrin antibodies generated against the two terminal extracellular Ig domains of recombinant human nephrin recognized a 180-kDa protein in lysates of human glomeruli and a 150-kDa protein in transfected COS-7 cell lysates.
Species: Human
Route: Synthetic Peptide. Immunogen: A synthetic peptide of human NPHS1
Calculated Molecular Weight: 135kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NPHS1 are readily searchable from our website. Different antibodies against the same target such as NPHS1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.