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anti-NPM antibody product blog

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Posted on 2017-03-04 01:32:59 by mybiosource_staff
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Tags: Antibody; anti-NPM antibody; NPM; Polyclonal Antibody; Nucleophosmin (NPM);
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The NPM npm1 (Catalog #MBS2002212) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Nucleophosmin (NPM) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s Nucleophosmin (NPM) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB).
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the NPM npm1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NPM npm1 product has the following accession number(s) (GI #6679108) (NCBI Accession #NP_032748.1) (Uniprot Accession #Q61937). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG SEF- DLKPSTPRS KGQESFKKQE KTPKTPKGPS SVEDIKAKMQ ASIEKGGSLP KVEAKFINYV KNCFRMTDQE AIQDLWQWRK SL.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Polyclonal Antibody to Nucleophosmin (NPM) with the following immunoassay(s):
Western Blot (WB) Knockout Verification (Knockout Verification: Lane 1: Wild-type Jurkat cell lysate; Lane 2: NPM knockout Jurkat cell lysate; Predicted MW: 33kd Observed MW: 38kd Primary Ab: 1ug/ml Rabbit Anti-Mouse NPM Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody )
Western Blot (WB) Knockout Verification NPM.

Western Blot (WB) (Western Blot; Lane1: Mouse Liver Tissue; Lane2: Human Jurkat Cells; Lane3: Human K562 Cells.)
Western Blot (WB) NPM.

Western Blot (WB) (Western Blot; Sample: Recombinant NPM, Mouse.)
Western Blot (WB) NPM.

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Pancreas Tissue.)
Immunohistochemistry (IHC) NPM.

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Adipose Tissue)
Immunohistochemistry (IHC) NPM.

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Mouse Intestine Tissue)
Immunohistochemistry (IHC) NPM.

Immunogen: Recombinant NPM (Asp212~Leu292) expressed in E.coli. Conjugated Antibody: The APC conjugated antibody version of this item is also available as catalog #MBS2067488. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NPM are readily searchable from our website. Different antibodies against the same target such as NPM may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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