anti-NRBP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; NRBP1; anti-NRBP1 antibody; NRBP;
The NRBP1 nrbp1 (Catalog #MBS624903) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The NRBP, NT (Nuclear Receptor Binding Protein, Nrbp1, BCON3, FLJ27109, FLJ35541, HLS7-interacting Protein Kinase, Multiple Domain Putative Nuclear Protein, MUDPNP, Myeloid Leukemia Factor 1 Adaptor Molecule, MLF1 Adapter Molecule, MADM) reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s NRBP can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC).Suitable for use in ELISA, Western Blot, and Immunohistochemistry.
Dilution: ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100. Researchers should empirically determine the suitability of the NRBP1 nrbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NRBP1 nrbp1 product has the following accession number(s) (GI #7019333) (NCBI Accession #NP_037524.1) (Uniprot Accession #Q9UHY1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the NRBP, NT (Nuclear Receptor Binding Protein, Nrbp1, BCON3, FLJ27109, FLJ35541, HLS7-interacting Protein Kinase, Multiple Domain Putative Nuclear Protein, MUDPNP, Myeloid Leukemia Factor 1 Adaptor Molecule, MLF1 Adapter Molecule, MADM) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of anti-NRBP Pab in HL-60 cell lysate (Lane 1) and mouse brain tissue lysate (Lane 2). NRBP (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)
Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)
Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. With more than 500 gene products, the protein kinase family is one of the largest families of proteins in eukaryotes. The family has been classified in 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains. The STE group (homologs of yeast Sterile 7, 11, 20 kinases) consists of 50 kinases related to the mitogen-activated protein kinase (MAPK) cascade families (Ste7/MAP2K, Ste11/MAP3K, and Ste20/MAP4K). MAP kinase cascades, consisting of a MAPK and one or more upstream regulatory kinases (MAPKKs) have been best characterized in the yeast pheromone response pathway. Pheromones bind to Ste cell surface receptors and activate yeast MAPK pathway.