Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Polyclonal Antibody Blog  rightarrow anti-NRP1 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-ASS1 antibody blog
 • anti-MYST-2 antibody blog
 • anti-a1BG antibody blog
 • anti-ACOT7 antibody blog
 • anti-C5 antibody blog
 • anti-FAP antibody blog
 • anti-CD70 antibody blog
 • anti-LRRC8A antibody blog
 • anti-MEF-2 antibody blog
 • anti-GLB1 antibody blog
 • anti-APOBEC3B antibody blog
 • anti-SOD2 antibody blog
 • anti-AIMP1 antibody blog
 • anti-CYP24 antibody blog
 • anti-Ndufa10 antibody blog
 • anti-USP39 antibody blog
 • anti-Rab27a antibody blog
 • anti-SARM antibody blog
 • anti-RAIDD, IN antibody blog
 • anti-Arrestin 1 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Complement Component 9 (C9) Antibody
 • TMEM59L Antibody
 • Macrophage colony-stimulating factor 1 receptor (CSF1R) Recombinant Protein
 • Serine-threonine-protein kinase NIM1 Antibody
 • MYOGLOBIN (MB) Antibody
 • PSA Antibody
 • c-abl oncogene 1, receptor tyrosine kinase (ABL1) ELISA Kit
 • Ig Fraction Immunoglobulin
 • IL-12 receptor beta 1 (IL12RB1) Recombinant Protein
 • Goat Anti-Human IgG, Monkey ads Secondary Antibody
 • Xanthine Dehydrogenase (XDH) ELISA Kit
 • Hypoxia Up Regulated 1 (HYOU1) ELISA Kit
 • Coiled-coil domain-containing protein 83 (CCDC83) ELISA Kit
 downarrow more ...

anti-NRP1 antibody product blog

Posted on 2015-08-23 12:53:16 by mybiosource_staff
Share it on ...     
Tags: Antibody; Polyclonal Antibody; anti-NRP1 antibody; NRP1; Neuropilin-1;
The NRP1 nrp1 (Catalog #MBS620404) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Neuropilin-1 (NRP1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (Carboxyfluorescein) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Neuropilin-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated on bEND.3 cells and PC-12 cells.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells
twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
4. The cells were resuspended in Flow Cytometry Staining
Buffer for analysis by Flow Cytometry. As a control for this
analysis, cells in a separate tube should be treated with
CFS-labeled goat IgG antibody. Researchers should empirically determine the suitability of the NRP1 nrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NRP1 nrp1 product has the following accession number(s) (GI #66912178) (NCBI Accession #NP_001019799.1) (Uniprot Accession #O14786). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted Semaphorin subfamily as well as some isoforms of VEGF family proteins. The aa sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.

NRP1 nrp1

Immunogen: Recombinant corresponding to rat Neuropilin-1, extracellular domain expressed in NSO cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NRP1 are readily searchable from our website. Different antibodies against the same target such as NRP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions