|The NRP1 nrp1 (Catalog #MBS620404) is an Antibody produced from Goat and is intended for research purposes only. The product is available for immediate purchase. The Neuropilin-1 (NRP1, BDCA4, CD304, DKFZp686A03134, DKFZp781F1414, NP1, NRP, Vascular endothelial cell growth factor 165 receptor, VEGF 165R) (Carboxyfluorescein) reacts with Rat and may cross-react with other species as described in the data sheet. MyBioSource\'s Neuropilin-1 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
Suitable for use in Flow Cytometry.
Dilution: Flow Cytometry: 10ul labels 1-2.5x10e5 cells. Validated on bEND.3 cells and PC-12 cells.
Flow Cytometry Protocol: 1. Cells may be Fc-blocked with 1ug of mouse IgG/10e5 cells for 15 minutes at RT. Do not wash excess blocking IgG from this reaction.
2. After blocking, 10ul of N1276-91C was added to 1-2.5x10e5 cells and incubated for 30 minutes at RT.
3. Unbound antibody was removed by washing the cells
twice in Flow Cytometry Staining Buffer. Note that whole blood requires a RBC lysis step at this point using Flow Cytometry Mouse Lyse Buffer.
4. The cells were resuspended in Flow Cytometry Staining
Buffer for analysis by Flow Cytometry. As a control for this
analysis, cells in a separate tube should be treated with
CFS-labeled goat IgG antibody. Researchers should empirically determine the suitability of the NRP1 nrp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The NRP1 nrp1 product has the following accession number(s) (GI #66912178) (NCBI Accession #NP_001019799.1) (Uniprot Accession #O14786). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Neuropilin-1 is a type I transmembrane protein that is expressed in the developing nervous system and by endothelial and tumor cells. Neuropilin-1 binds members of the class III secreted Semaphorin subfamily as well as some isoforms of VEGF family proteins. The aa sequence of rat Neuropilin-1 extracellular domain is 98% and 93% identical to that of mouse and human Neuropilin-1, respectively.
Immunogen: Recombinant corresponding to rat Neuropilin-1, extracellular domain expressed in NSO cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NRP1 are readily searchable from our website. Different antibodies against the same target such as NRP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.