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anti-NTRK1 antibody product blog

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Posted on 2018-02-28 22:34:26 by mybiosource_staff
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Tags: Antibody; Polyclonal Antibody; TrkA; NTRK1; anti-NTRK1 antibody;
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The NTRK1 ntrk1 (Catalog #MBS125190) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The TrkA Rabbit pab Antibody reacts with Human, mouse, rat and may cross-react with other species as described in the data sheet. MyBioSource\'s TrkA can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunoprecipitation (IP), Flow Cytometry (FC/FACS).
Western Blot (WB): 1/1000-2000
Immunohistochemistry (IHC): 1/50
Immunocytochemistry (ICC): 1/50
Immunoprecipitation (IP): 1/20
Flow Cytometry (FC/FACS): 1/10. Researchers should empirically determine the suitability of the NTRK1 ntrk1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The NTRK1 ntrk1 product has the following accession number(s) (GI #56118210) (NCBI Accession #NP_001007793.1) (Uniprot Accession #P04629). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the TrkA Rabbit pab Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of rat brain, using NTRK1 antibody.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Western Blot (WB) NTRK1.

Rabbit polyclonal to TrkA

The family of Trk receptor tyrosine kinases consists of TrkA, TrkB and TrkC. While the sequence of these family members is highly conserved, they are activated by different neurotrophins: TrkA by NGF, TrkB by BDNF or NT4, and TrkC by NT3. TrkA regulates proliferation and is important for development and maturation of the nervous system (1). Phosphorylation at Tyr490 is required for Shc association and activation of the Ras-MAP kinase cascade. Residues Tyr674/675 lie within the catalytic domain, and phosphorylation at this site reflects TrkA kinase activity (2-6). Point mutations, deletions and chromosomal rearrangements (chimeras) cause ligand-independent receptor dimerization and activation of TrkA. Many malignancies including breast, colon, prostate and thyroid carcinomas and acute myeloid leukemia have activated TrkA. Expression of TrkA in neuroblastomas is a good prognostic marker because it signals growth arrest and differentiation of cells originating from the neural crest (1).

Immunogen: A synthetic -peptide of human TrkA. Buffer: PBS with 0.1% Sodium Azide, 50% Glycerol,. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing NTRK1 are readily searchable from our website. Different antibodies against the same target such as NTRK1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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