|The ON sparc (Catalog #MBS2005691) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to Osteonectin (ON) reacts with Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s Osteonectin (ON) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) Formalin/Parafin, ELISA (EIA), Western Blot (WB).
Western blotting: 1:100-400
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the ON sparc for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The ON sparc product has the following accession number(s) (GI #338325) (NCBI Accession #AAA60993.1) (Uniprot Accession #P09486). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful. The amino acid sequence is listed below:
The target protein is fused with N-terminal His-Tag and its sequence is listed below.
MGHHHHHHSG S-APQ QEALPDETEV VEETVAEVAE VPVGANPVQV EVGEFDDGAE ETEEEVVAEN PCQNHHCKHG KVCELDENNT PMCVCQDPTS CPAPIGEFEK VCSNDNKTFD SSCHFFATKC TLEGTKKGHK LHLDYIGPCK YIPPCLDSEL TEFPLRMRDW LKNVLVTLYE RDEDNNLLTE KQKLRVKKIH ENEKRLEAGD HPVELLARDF EKNYNMYIFP VHWQFGQLDQ HPIDGYLSHT ELAPLRAPLI PMEHCTTRFF ETCDLDNDKY IALDEWAGCF GIKEKDIDKD LVI.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Immunogen: Recombinant ON (Ala18~Ile303) expressed in E.coli. Conjugation: No Conjugate. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing ON are readily searchable from our website. Different antibodies against the same target such as ON may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. ON also interacts with the following gene(s): COL1A1, EGF, FN1, PLG, SERPINE1, TGFB1, TIMP1. The following patways have been known to be associated with this gene. Blood, Bone, Bone Marrow, Brain, Connective Tissue, Embryonic Tissue, Mouth, Muscle, Skin, Vascular tissues are correlated with this protein. Adenocarcinoma, Brain Diseases, Breast Neoplasms, Cardiovascular Diseases, Fibrosis, Heart Diseases, Inflammation, Necrosis, Neoplasms, Neoplasms, Experimental are some of the diseases may be linked to Polyclonal Antibody to Osteonectin (ON).