anti-OTOP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; OTOP1; anti-OTOP1 antibody;
The OTOP1 otop1 (Catalog #MBS9205488) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The OTOP1 Antibody (Center) reacts with Mouse (Predicted Reactivity: Rat) and may cross-react with other species as described in the data sheet. MyBioSource\'s OTOP1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC).WB~~1:1000
IHC-P: 1:10~50. Researchers should empirically determine the suitability of the OTOP1 otop1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The OTOP1 otop1 product has the following accession number(s) (GI #29725635) (NCBI Accession #NP_819056.1) (Uniprot Accession #Q7RTM1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the OTOP1 Antibody (Center) with the following immunoassay(s):
Western Blot (WB) (OTOP1 Antibody (Center) western blot analysis in mouse heart tissue lysates (35ug/lane).This demonstrates the OTOP1 antibody detected the OTOP1 protein (arrow).)
Immunohistochemistry (IHC) (OTOP1 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of OTOP1 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)
OTOP1 is required for normal formation of otoconia in the inner ear. Inhibits P2Y purinoceptors. Modulates calcium homeostasis and influx of calcium in response to extracellular ATP (By similarity).
Cellular Location: Cell membrane {ECO:0000250|UniProtKB:Q80VM9};Multi-pass membrane protein. Note=Detectedin the gelatinous membrane overlying theinner ear macular epithelium.{ECO:0000250|UniProtKB:Q80VM9
Function: Proton-selective channel that specificallytransports protons into cells (PubMed:29371428). Protonchannel activity is only weakly-sensitive tovoltage (By similarity). Proton-selectivechannel activity is probably required in celltypes that use changes in intracellular pH forcell signaling or to regulate biochemical ordevelopmental processes (PubMed:29371428). In thevestibular system of the inner ear, requiredfor the formation and function of otoconia,which are calcium carbonate crystals thatsense gravity and acceleration (By similarity).Probably acts by maintaining the pHappropriate for formation of otoconia (Bysimilarity). Regulates purinergic control ofintracellular calcium in vestibular supportingcells (By similarity). May be involved in sourtaste perception in sour taste cells bymediating entry of protons within the cytosol(By similarity). Also involved in energymetabolism, by reducing adipose tissueinflammation and protecting fromobesity-induced metabolic dysfunction (Bysimilarity). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing OTOP1 are readily searchable from our website. Different antibodies against the same target such as OTOP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.