anti-P4HB antibody product blog
Tags: Antibody; Polyclonal Antibody; P4HB; anti-P4HB antibody;
The P4HB p4hb (Catalog #MBS126305) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The P4HB Polyclonal Antibody reacts with Human, Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s P4HB can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunoprecipitation (IP), RIP.WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:20 - 1:100
IP: 1:20 - 1:50
RIP: 1:20 - 1:50. Researchers should empirically determine the suitability of the P4HB p4hb for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The P4HB p4hb product has the following accession number(s) (GI #2507460) (NCBI Accession #P07237.3) (Uniprot Accession #P07237). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the P4HB Polyclonal Antibody with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts of various cell lines, using P4HB antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (MBS128200) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.)
Immunofluorescence (IF) (Immunofluorescence analysis of HeLa cells using P4HB antibody. Blue: DAPI for nuclear staining.)
Immunoprecipitation (IP) (Immunoprecipitation analysis of 200ug extracts of SW620 cells using 3ug P4HB antibody. Western blot was performed from the immunoprecipitate using P4HB antibody at a dilition of 1:500.)
During their synthesis, secretory proteins translocate into the endoplasmic reticulum (ER) where they are post-translationally modified and properly folded. To reach their native conformation, many secretory proteins require the formation of intra- or inter-molecular disulfide bonds (1). This process is called oxidative protein folding. Protein disulfide isomerase (PDI) catalyzes the formation and isomerization of these disulfide bonds (2). Studies on mechanisms of oxidative folding suggest that molecular oxygen oxidizes the ER-protein Ero1, which in turn oxidizes PDI through disulfide exchange (3). This event is then followed by PDI-catalyzed disulfide bond formation in folding proteins (3).
Species: Human
Immunogen: Recombinant Protein. Immunogen: Recombinant protein of human P4HB
Calculated Molecular Weight: 57kDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing P4HB are readily searchable from our website. Different antibodies against the same target such as P4HB may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.