anti-p53 Binding Protein 1 antibody product blog
Tags: Antibody; Polyclonal Antibody; p53 Binding Protein 1; anti-p53 Binding Protein 1 antibody;
The p53 Binding Protein 1 n/a (Catalog #MBS623476) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The p53 Binding Protein 1, phosphorylated (Ser25/29) (53BP1, 53-BP1, FLJ41424, MGC138366, p202, p53BP1, Tumor Protein 53 Binding Protein 1, Tumor Protein p53 Binding Protein 1, TP53BP1, Tumor Suppressor p53 Binding Protein 1) reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s p53 Binding Protein 1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB).Suitable for use in Western Blot. Researchers should empirically determine the suitability of the p53 Binding Protein 1 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The p53 Binding Protein 1 n/a product has the following accession number(s) (GI #1842230) (NCBI Accession #AAC53040.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the p53 Binding Protein 1, phosphorylated (Ser25/29) (53BP1, 53-BP1, FLJ41424, MGC138366, p202, p53BP1, Tumor Protein 53 Binding Protein 1, Tumor Protein p53 Binding Protein 1, TP53BP1, Tumor Suppressor p53 Binding Protein 1) with the following immunoassay(s):
Western Blot (WB) (Western blot analysis of extracts from 293 cells, untreated or UV-treated (50 mJ for 2 hours), using Phospho-53BP1 (Ser25/29) Antibody (upper) or 53BP1 Antibody (lower).)
p53-binding protein 1 (53BP1) was originally identified as a p53 binding partner that could enhance the transcriptional activity of p53 (1,2). 53BP1 consists of two BRCA1 carboxy terminal (BRCT) domains that allow for binding to p53 and a separate domain responsible for binding to phosphorylated histone H2A.X (3). 53BP1 rapidly translocates to nuclear foci following treatment of cells with ionizing radiation (IR) or radiomimetic agents that cause DNA double strand breaks (DSBs) (4,5). Because of this localization to DSBs and homology to the yeast protein Rad9, a role for 53BP1 in DSB repair has been proposed. Recruitment of 53BP1 to sites of DNA damage has been demonstrated to be independent of ATM, NBS1, and DNA-PK (4) and retention of 53BP1 at DNA breaks requires phosphorylated H2A.X (6). In cells lacking 53BP1, phosphorylation of ATM substrates is reduced, suggesting that 53BP1 is upstream of ATM (7). In response to IR, phosphorylation of 53BP1 at serines 6, 25, 29, and 784 by ATM has been demonstrated, but phosphorylation at these sites is not required for localization of 53BP1 to sites of DSBs (6).