anti-PARP1 antibody product blog
Tags: Antibody; Polyclonal Antibody; PARP1; anti-PARP1 antibody; PARP, Cleavage Site;
The PARP1 parp1 (Catalog #MBS614355) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s PARP, Cleavage Site can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FC/FACS), Immunofluorescence (IF).Suitable for use in ELISA, Western Blot, Immunohistochemistry, Immunofluorescence and Flow Cytometry.
Dilution: Western Blot: 1:1000
Immunohistochemistry (Paraffin): 1:50
Immunocytochemistry (IF): 1:200
Flow Cytometry: 1:25. Researchers should empirically determine the suitability of the PARP1 parp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PARP1 parp1 product has the following accession number(s) (GI #156523968) (NCBI Accession #NP_001609.2) (Uniprot Accession #P09874). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase) with the following immunoassay(s):
Western Blot (WB) (Western Blot analysis of Jurkat cells, untreated or etoposide-treated (25µM), using MBS614355.)
Testing Data
Testing Data
Testing Data
PARP, a 116kD nuclear poly (ADPribose) polymerase, appears to be involved in DNA repair predominantly in response to environmental stress (1). This protein can be cleaved by many ICElike caspases in vitro (2,3) and is one of the main cleavage targets of caspase-3 in vivo (4,5). In human PARP the cleavage occurs between Asp214 and Gly215, which separates PARP\'s N-terminal DNA binding domain (24kD) from its C-terminal catalytic domain (89kD) (2,4). PARP is important for cells to maintain their viability; cleavage of PARP facilitates cellular disassembly and serves as a marker of cells undergoing apoptosis (6).
Immunogen: Synthetic peptide (KLH coupled) corresponding to C-terminal residues adjacent to Asp214 in human PARP. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PARP1 are readily searchable from our website. Different antibodies against the same target such as PARP1 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Brain, Kidney, Liver, Lung, Muscle, Nerve, Prostate, Skin, Vascular tissues are correlated with this protein. Breast Neoplasms, Inflammation, Liver Diseases, Lung Neoplasms, Necrosis, Neoplasms, Neoplasms, Experimental, Nervous System Diseases, Ovarian Neoplasms, Prostatic Neoplasms are some of the diseases may be linked to PARP, Cleavage Site (Asp214) (Poly ADP-Ribose Polymerase).