anti-PARPBP antibody product blog
Tags: Antibody; Polyclonal Antibody; C12orf48; anti-PARPBP antibody; PARPBP;
The PARPBP parpbp (Catalog #MBS9215174) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The C12orf48 Antibody (C-term) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s C12orf48 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).WB~~1:1000
IHC-P~~1:50~100
FC~~1:10~50. Researchers should empirically determine the suitability of the PARPBP parpbp for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The PARPBP parpbp product has the following accession number(s) (GI #300797388) (NCBI Accession #NP_060385.3) (Uniprot Accession #Q9NWS1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the C12orf48 Antibody (C-term) with the following immunoassay(s):
Western Blot (WB) (C12orf48 Antibody (C-term)(MBS9215174) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the C12orf48 antibody detected the FBXO34 protein (arrow).)
Immunohistochemistry (IHC) (C12orf48 antibody (C-term)(MBS9215174) immunohistochemistry analysis in formalin fixed and paraffin embedded human lymph tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the C12orf48 antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)
Flow Cytometry (FC/FACS) (C12orf48 Antibody (C-term)(MBS9215174) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Function: Required to suppress inappropriate homologous recombination, thereby playing a central role DNA repair and in the maintenance of genomic stability. Antagonizes homologous recombination by interfering with the formation of the RAD51-DNA homologous recombination structure. Binds single-strand DNA and poly(A) homopolymers. Positively regulate the poly(ADP-ribosyl)ation activity of PARP1; however such function may be indirect.
Cellular Location: Cytoplasm. Nucleus Note=Localizes to chromatin in response to S phase arrest but not in mitosis. Targeted to chromatin via its interaction with PCNA
Tissue Location: Restricted to testis. Overexpressed in multiple cancer cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing PARPBP are readily searchable from our website. Different antibodies against the same target such as PARPBP may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.